Laboratory for Biology of Secondary Metabolism

Ing. Jiří Janata, CSc.

 

 

 

Head of the Laboratory: Dipl. Ing. Jiri Janata, PhD.

Phone: +420 296 442 370
E-mail: janata@biomed.cas.cz

 

 

Ing. Jiří Janata, CSc. Vedoucí laboratoře
Mgr. Lucie Najmanová, Ph.D. Vědecký pracovník
Ing. Radek Gažák , PhD Vědecký pracovník
Mgr. Zdeněk Kameník , Ph.D. Postdoc
Mgr. Stanislav Kadlčík , Ph.D. Postdoc
Ing. Anna Gažáková PhD student
Mgr. Lenka Sabová PhD student
Mgr. Petra Jirásková PhD student
Mgr. Magdalena Pavlíková PhD student
Mgr. Lucie Steiningerová PhD student
Mgr. Šimon Vobruba PhD student
Miloslav Klégr technický pracovník
Mgr. Natalia Slonská technický pracovník
Bc. Barbora Tešínská student
Ing. Lucie Palánová PhD student
Ing. Markéta Válková PhD student
Bc. Lukáš Cudlman Student
Bc. Lada Brázdová Student
Mgr. Gabriela Novotná Balíková, Ph.D. Vedoucí skupiny
RNDr.  Markéta Koběrská, Ph.D. Vědecký pracovník
Vladimir Vimberg , Ph.D. Postdoc
Mgr. Jakub Lenart PhD student
Mgr. Ludmila Veselá PhD student
Mgr. Leona Zieglerová PhD student
Mgr.  Jana Veselá technický pracovník
Bc. Nikola Pinďáková Student
Bc. Lenka Nguyen Student
Bc. Jana Prášilová Student

The main research topic of the laboratory is the biosynthesis of secondary metabolites. In other words, we study how the bacteria produce biologically active compounds like for example antibiotics (in our case lincomycin – a drug clinically used for example in stomatology) or anticancer pyrrolobenzodiazepines. The detailed knowledge of biosynthetic pathways is the first prerequisite for their targeted modifications in order to produce new, more efficient compounds.

This topic is tightly connected with our second aim – the study of mechanisms of action of antibiotics and, from bacterial point of view, the mechanisms of resistance. Besides the above mentioned lincosamides (lincomycin in our case) in this project we study also macrolides (for example erythromycin), streptogramins (chinupristin/dalfopristin) and glycopeptides (vancomycin and teicoplanin). The clinical isolates of staphylococci from Czech hospitals serve as model to study the mode of antibiotic action and mechanisms of resistance.

The modern methods of high throughput DNA sequencing are used in our lab to search for producers of new biologically active compounds with biotechnological potential from the environment. We also use these methods to study the correlation of bacterial communities’ composition with the environmental pollution (the monitoring of waste water treatment plants efficiency for example).

Another application of high throughput sequencing methods is our study of the composition of oral bacterial communities in patients with periodontits, a disease that threatens over 30% of world population. We investigate the changes in composition of oral bacterial consortia based on the clinical state of patients and the treatment. The aim of the project is to evolve more efficient system for monitoring of the onset of the periodontal disease and its treatment (including the response to the antibiotic therapy). The better monitoring system will enable to identify patients in risk of periodontits and to start efficient prophylaxis resulting in the decrease of treatment costs.

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