kyslikHead of the Laboratory: Pavel Kyslík, PhD.

Phone : +420 241 062 777




Laboratory of Enzyme Technology has been established in 1993 as a part of a Division of Biogenesis and Biotechnology of Nature Compounds, Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic. The laboratory is engaged in research & development projects of a multi-disciplinary character encompassing microbiology, molecular biology, biochemistry, bioengineering and biotechnology. The laboratory meets requirements for the work with GMO.

The main research interest of the laboratory is aimed at:

  • Bioprospecting and construction of the recombinant microorganisms with novel metabolic capabilities
  • Characterization of novel enzymes and microbial products
  • Construction of enzymes with optimal traits by rational design and directed evolution
  • The improvement of microorganisms and construction of production strains
  • Stabilization of intact microorganism or enzymes and development of catalysts for biocatalysis of nature compounds or chemical substances
  • Development of bioprocesses up to a pilot scale (up-stream and down-stream processes)

Current research topics:

  • Bacterial siderophores
  • Biosynthetic potential and host-plasmid interactions in enzyme overproducing microorganisms
  • Enantioselective epoxide hydrolases
  • Catalysts for biotransformation of β-lactam antibiotics
  • Pyranose 2-oxidase: biocatalysis of saccharides
  • Laboratory culture collection of microorganisms


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