Head of the Laboratory: Pavel Kyslík, PhD.
Phone : +420 241 062 777
Email: kyslik@biomed.cas.cz
Laboratory of Enzyme Technology has been established in 1993 as a part of a Division of Biogenesis and Biotechnology of Nature Compounds, Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic. The laboratory is engaged in research & development projects of a multi-disciplinary character encompassing microbiology, molecular biology, biochemistry, bioengineering and biotechnology. The laboratory meets requirements for the work with GMO.
The main research interest of the laboratory is aimed at:
- Bioprospecting and construction of the recombinant microorganisms with novel metabolic capabilities
- Characterization of novel enzymes and microbial products
- Construction of enzymes with optimal traits by rational design and directed evolution
- The improvement of microorganisms and construction of production strains
- Stabilization of intact microorganism or enzymes and development of catalysts for biocatalysis of nature compounds or chemical substances
- Development of bioprocesses up to a pilot scale (up-stream and down-stream processes)
Current research topics:
- Bacterial siderophores
- Biosynthetic potential and host-plasmid interactions in enzyme overproducing microorganisms
- Enantioselective epoxide hydrolases
- Catalysts for biotransformation of β-lactam antibiotics
- Pyranose 2-oxidase: biocatalysis of saccharides
- Laboratory culture collection of microorganisms